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All Journal JURNAL ILMIAH KESEHATAN KEPERAWATAN Jurnal Kesehatan Reproduksi Indonesian Journal of Biotechnology Jurnal Kesehatan Kesmas Indonesia: Jurnal Ilmiah Kesehatan Masyarakat Jurnal Kesehatan Ibu dan Anak JFIOnline YARSI Medical Journal Jurnal Biomedika dan Kesehatan Jurnal Ilmiah PANNMED (Pharmacist, Analyst, Nurse, Nutrition, Midwivery, Environment, Dentist)
Djaswadi Dasuki
Departemen Obstetri dan Ginekologi Rumah Sakit Umum Sardjito Yogyakarta
Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health

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Journal : YARSI Medical Journal

 1 
Pengaruh Kurkumin pada Kultur Sel Luteal Tikus yang mengandung Teofilin terhadap Kadar cAMP dan Progesteron Purwaningsih, Endang; Kadarsih Soejono, Sri; Dasuki, Djaswadi; Meiyanto, Edy
Jurnal Kedokteran YARSI Vol 17, No 3 (2009): SEPTEMBER - DESEMBER 2009
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (286.417 KB) | DOI: 10.33476/jky.v17i3.210

Abstract

Senyawa kurkumin dapat menghambat steroidogenesis kultur sel luteal dengan menghambat sekresi progesteron. Letak kerja kurkumin pada steroidogenesis kultur sel luteal belum diketahui. Penelitian ini dimaksudkan untuk mengetahui pengaruh kurkumin terhadap kadar cAMP dan kadar progesteron pada steroidogenesis kultur sel luteal dengan penambahan teofilin. Subyek penelitian adalah korpus luteum tikus Sprague Dawley yang diinduksi dengan PMSG. Kurkumin diberikan sesaat setelah stimulasi LH dan atau PGF2a dengan dan tanpa penambahan teofilin. Kemudian kultur sel diinkubasi selama 24 jam. Konsentrasi cAMP diukur dengan metode ELISA sedangkam konsentrasi progesteron diukur dengan metode RIA. Hasil penelitian menunjukkan bahwa LH meningkatkan cAMP dan kadar progesteron secara bermakna, sedangkan PGF2a mengurangi kadar cAMP dan kadar progesteron secara tidak bermakna. Teofilin meningkatkan kadar cAMP dan kadar progesteron secara bermakna dan hampir sama dengan stimulasi LH. Kurkumin menghambat kadar cAMP oleh LH maupun teofilin. Disimpulkan bahwa kurkumin menghambat kadar cAMP dan kadar progesteron pada kultur sel luteal dengan cara menekan transduksi sinyal di up stream cAMP.
Efek Pentagamavunon-0 terhadap Konsentrasi cAMP dan Progesteron pada Kultur Sel Luteal yang mengandung Teofilin Purwaningsih, Endang; Kadarsih Soejono, Sri; Dasuki, Djaswadi; Meiyanto, Edy
Jurnal Kedokteran YARSI Vol 17, No 2 (2009): MEI - AGUSTUS 2009
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33476/jky.v17i2.202

Abstract

Curcumin analog (Pentagamavunon-0/PGV-0) can inhibit steroidogenesis of luteal cell culture. Corpus luteum secretes progesterone by LH stimulation. The main transduction signal of luteal cells steroidogenesis is through the cAMP/PKA. The objective of this study was to know the effect of PGV-0 on cAMP and progesterone concentration of luteal cell culture containing theophylline. The subject was corpus luteum of rat Sprague Dawley strain induced with PMSG (10 IU). PGV-0 was given shortly after the stimulation of LH and or PGF2? with or without theophyline. The cell culture then put into the incubator for 24 hours. Concentration of cAMP was assessed by ELISA whereas the progesterone concentration was determined by RIA. The result showed that LH stimulation caused cAMP and progesterone increase significantly. The inhibition of PGF2? on cAMP and progesterone concentrations showed no significant difference compared to the control. Theophylline increased the cAMP and progesterone concentration significantly but not to LH stimulation. PGV-0 did not inhibit cAMP concentration but PGV-0 inhibited the progesterone concentration by LH stimulation. In conclusion, PGV-0 inhibits signal transduction of lutheal cell in down stream cAMP.
EFEK KURKUMIN SINTESIS DAN PENTAGAMAVUNON-0 TERHADAP PRODUKSI PROGESTERON KULTUR SEL LUTEAL DENGAN PEMBERIAN FORSKOLIN Purwaningsih, Endang; Meiyanto, Edy; Dasuki, Djaswadi; Kadarsih Soejono, Sri
Jurnal Kedokteran YARSI Vol 15, No 3 (2007): SEPTEMBER-DESEMBER 2007
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.724 KB) | DOI: 10.33476/jky.v15i3.1075

Abstract

Curcumin is one of the common traditional medicines that is often used for fertility regulation. Curcumin analogue, pentagamavunon-0/PGV-0, exerted similar effect with curcumin. Synthetic curcumin and PGV-0 possesses molecular structures similar to prostaglandin. It has been reported that luteal cells are able to produce progesterone in vitro by addition of several hormones such as LH and prostaglandin F2? (PGF2?). Stimulation of luteal cell cultures with LH and/or PGF2? in the presence of synthetic curcumin and PGV-0 will interfere with the progesterone production. Nevertheless, the precise site of action of curcumin remains unknown. The present study aims to determine the effect of synthetic curcumin and PGV-0 on the progesterone production by luteal cell cultures in the presence or absence of several known stimulators or inhibitors sucs as LH, PGF2? and forskolin, respectively. The results indicated that synthetic curcumin reduced the progesterone concentration significantly (p 0,05) in the luteal cell culture treated with solvent, LH and/or PGF2?, in the presence or absence of forskolin. In the groups treated with PGV-0, and induced with LH and/or PGF2?, the concentration of progesterone did not change significantly (p 0,05). Addition of forskolin on the control group resulted in higher progesterone concentration as that of LH. The findings indicated that synthetic curcumin inhibits progesterone production by the luteal cell cultures through the cAMP/PKA/MAP-Kinase signaling cascade, but PGV-0 might have the site of action in the PLC/PKC/MAP-Kinase signaling cascade. 
Co-Authors Ade Ana Adelina Amelia Agustin Endriyani, Agustin Ahmad Hamim Sadewa Andriana Kumala Dewi Arief Boediono Asmar Yetty Zein, Asmar Yetty Attamimi, Ahsanudin Banun Kusumawardani Batubara, Irwan Dameria, Netty Katrina Dewi, Adinda Putri Sari Dewi, Fovilia Dewi, Suryani Puspa Dhesi Ari Astuti, Dhesi Ari Diah Rumekti Diah Rumekti Hadiati Dita Maria Virginia Djauhar Ismail Edy Meiyanto Endang Purwaningsih Ery purwanti Heru Pradjatmo Husnawati Husnawati Irwan Taufiqurahman Ita Fauzia Hanoum, Ita Fauzia Kadarsih Soejono, Sri Kadarsih Soejono, Sri Kadek Yuke Widyantari Kartini, Farida Kora, Firmina Th Lamana, Aspia Legawati Legawati, Legawati Madarina Julia Mardiana Mardiana Marga, Anik Dwi Marsetyawan Soesatyo Marta Isyana Menik Sri Daryanti, Menik Sri Mubasysyir Hasanbasri MULYOTO PANGESTU Mustofa Mustofa Nurhaya S Patui Ova Emilia Pinandari, Anggriyani W. Pinda Hutajulu, Pinda Putri, Nita Tri R. Sapto Hendri Boedi Soesatyo Retno Mawarti, Retno Rukmono Siswishanto Shofwal Widad Siswanto Agus Wilopo Sri Handayani Sri Kadarsih S, Sri Sulchan Sofoewan, Sulchan Sumarni DW, Sumarni SUMIYATI SUMIYATI Surjadi, Lily Marliany Susilawati, Budi Totok Utoro Tri Hastuti Tunjung Wibowo Virarisca, Sheilla Wahyuni, Budi Widya Asmara Yayi Suryo Prabandari Yundari, Yundari